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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
05/10/2011 |
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Data da última atualização: |
10/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E. |
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Afiliação: |
INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, SAPC; UNIVERSIDADE FEDERAL DO PARANÁ; UNIVERSIDADE FEDERAL DO PARANÁ. |
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Título: |
Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Environmental and Experimental Botany, v.72, n. 2, p. 242-250, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
In this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress. MenosIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was m... Mostrar Tudo |
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Palavras-Chave: |
Citrus rootstock; Osmotic adjustment. |
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Thesaurus Nal: |
Drought tolerance; Oxidative stress; Proline. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42905/1/Drought-tolerance-and-antioxidant.pdf
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Marc: |
LEADER 02726naa a2200253 a 4500
001 1902435
005 2024-01-10
008 2011 bl uuuu u00u1 u #d
100 1 $aCAMPOS, M. K. F. de
245 $aDrought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline.$h[electronic resource]
260 $c2011
520 $aIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress.
650 $aDrought tolerance
650 $aOxidative stress
650 $aProline
653 $aCitrus rootstock
653 $aOsmotic adjustment
700 1 $aCARVALHO, K. de
700 1 $aSOUZA, F. S. de
700 1 $aMARUR, C. J.
700 1 $aPEREIRA, L. F. P.
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aVIEIRA, L. G. E.
773 $tEnvironmental and Experimental Botany$gv.72, n. 2, p. 242-250, 2011.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
12/01/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ALENCAR, M. de C. F.; ABREU, L. S. de. |
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Afiliação: |
MARIA DE CLEOFAS FAGGION ALENCAR, CNPMA; LUCIMAR SANTIAGO DE ABREU, CNPMA. |
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Título: |
A desconstrução do imaginário da relação mulher - terra e sustentabilidade. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESO LATINOAMERICANO DE SOCIOLOGIA RURAL, 8., 2010, Porto de Galinhas. América Latina: realineamentos políticos y proyetos en disputa. Porto de Galinhas: ALASRU: UFRPE, 2010. 1 CD-ROM. |
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Páginas: |
9 p. |
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Idioma: |
Português |
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Thesagro: |
Agricultura Familiar; Agricultura Sustentável. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00619naa a2200157 a 4500
001 1872770
005 2011-01-12
008 2010 bl --- 0-- u #d
100 1 $aALENCAR, M. de C. F.
245 $aA desconstrução do imaginário da relação mulher - terra e sustentabilidade.
260 $c2010
300 $a9 p.
650 $aAgricultura Familiar
650 $aAgricultura Sustentável
700 1 $aABREU, L. S. de
773 $tIn: CONGRESO LATINOAMERICANO DE SOCIOLOGIA RURAL, 8., 2010, Porto de Galinhas. América Latina: realineamentos políticos y proyetos en disputa. Porto de Galinhas: ALASRU: UFRPE, 2010. 1 CD-ROM.
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